In this example, we evolve a sequence that stabilizes the structure of the PDZ domain of Rho guanine nucleotide exchange factor 12 (PDB-ID 2OMJ). We also show how to analyze the Simulated Annealing trajectory as well as how to additionally verify the quasi-stability of the artificially evolved sequence. We assume that eVolver and other required programs were installed as described in the installation instructions.

© Michal Brylinski
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